Maximum chance phylogenetic relationships of control area…

Maximum chance phylogenetic relationships of control area…

Optimum chance phylogenetic relationships of control area haplotypes based on market bought eyeball examples and sequences obtained from understood individuals.

Phylogenetic relationships had been identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony size = 141). Help values listed above nodes are Bayesian probabilities according to 5,000,000 resamples, maximum chance bootstrap help values predicated on 1,000 bootstrap replicates, and maximum parsimony bootstrap help values centered on 2,000 bootstrap replicates. Help values are shown limited to clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and also for the monophyly of Sotalia. Branch lengths are proportional to likelihood that is maximum divergence; scale shows percentage of series divergence. Haplotypes 1–14, highlighted in bold, had been present in our correspond and study to GenBank accession #EU022531–EU022544.

To advance confirm the relationships regarding the unknown sequences, we performed a nonphylogenetic cladistic analysis. We constructed a matrix of autapomorphies for Sotalia fluviatilis, Sotalia guianensis, Inia g. Geoffrensis, Inia g. Humboltiana, and Inia g. Boliviensis predicated on available sequence information in Genbank and our guide individuals, and completed a populace aggregation analysis ( Davis and Nixon 1992 ).

All eyeball?derived sequences decided by BLAST to be of cetacean origin shared species?specific autapomorphic character states indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis. The control area and Cytochrome b information sets have two and six diagnostic web web web sites, respectively, that assign all eyeball that is cetacean to Sotalia guianensis and unambiguously differentiate it from Sotalia fluviatilis and Inia spp. ( dining Table 1; online Appendix S1, S2).

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Control area BEL MAO PVH
Variable position 39 136 139 160 175 231 256 269 270 362 381 387 404 463 504 559 Haplotype occurence
Inia spp. A T the G C G G/A A A T C C/T C/T C C/T A ? ? ?
Sotalia fluviatilis T T A G C/T G C/T T C C/T C T T C T A ? ? ?
Sotalia guianensis C G/T A/C A C/T G/A C/T C/T C/T C/T C/G C/T C/T C/T C/T C/A 22 11 1
CR_Hap01 C T A A T A C C C C C T C C T the 1 ? ?
CR_Hap02 C T A A T A C T C C C C T C T A 2 ? ?
CR_Hap03 C T A A T A C C C C C C T C T the 6 2 ?
CR_Hap04 C T A A T A C T C C C T C C T A 3 5 1
CR_Hap05 C T A A T A C T C T C T C C T the 1 2 ?
CR_Hap06 C T the A C the T T T C C T T C T the 1 ? ?
CR_Hap07 C T A A T A C T C C C C C C T A 3 ? ?
CR_Hap08 C G C A T G C T C C C T C C T the 1 ? ?
CR_Hap09 C T A A T G C T C C C T C C T the 1 ? ?
CR_Hap10 C T A A T A C C C C C T T C T the 1 ? ?
CR_Hap11 C T A A T A C C C C C C T T T A ? 1 ?
CR_Hap12 C T A A T A C C C C C C T C T C ? 1 ?
CR_Hap13 C T the A C the C T T C C T T C C the 1 ? ?
CR_Hap14 C T A A T A C C C C G C T C T the 1 ? ?
Cytochrome b
adjustable position 49 78 124 297 367 396 462 501 528 531 561
Inia spp. G T A C G C T T A C T ? ? ?
Sotalia fluviatilis G C A G G C T the A C T ? ? ?
Sotalia guianensis G/A C/T G/A A G/A C/T C G C T C 16 7 1
CB_Hap01 G C A A G C C G C T C 8 4 1
CB_Hap02 A C A the G C C G C T C 1 ? ?
CB_Hap03 G T A A G C C G C T C 1 ? ?
CB_Hap04 G C G A G T C G C T C 3 1 ?
CB_Hap05 G C G A A T C G C T C 3 2 ?
  • Web Sites highlighted in bold unambiguously assign the unknown eyeball examples to Sotalia guianensis. Those maybe perhaps maybe not highlighted differentiate noticed haplotypes. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.

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